Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio250G
BICINE BioXtra 99 titratio250G
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer Bi
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This SOLUTIONon is effective under slightly basic conditions which keeps DNA deprotonated water-soluble and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
DULBECCOS Dulbeccos Phosphate Buffer
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Tris-Glycine-SDS Buffer 101L
Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffer Solution | 14265-44-2 | MFCD00145108 | 1 gal
Phosphate Buffer Solution | Mol Wt: 94.97 | 14265-44-2 | MFCD00145108 | 1 gal
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML
MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML
IBA LifeSciences Buffer XT-R - Strep-TactinXT regeneration buffer; 250 ml
Buffer XT-R is a ready-to-use Strep-TactinXT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-TactinXT after the elution of Strep-tagII or Twin-Strep-tag fusion proteins. Please note that Buffer XT-R is not applicable for Strep-Tactin.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer Bi1L
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This SOLUTIONon is effective under slightly basic conditions which keeps DNA deprotonated water-soluble and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi500G
PIPES BioPerformance Certi500G
Teknova 10X PBS with 0.5% Tween 20. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova PPB Solution. 1000mL, Sterile.
30mM Tris-HCl pH 8.0, 1mM Ethylenediaminetetraacetic acid (EDTA) pH 8.0,20% Sucrose